Table 2.
Primers used in this work.
| Primer | Sequence∗ | Purpose |
|---|---|---|
| PAampG-F | 5′GGGATCCCAACGCGCACGCTTGCGCGAGGA 3′(BamHI) | Cloning of ampG of PA01 |
| PAampG-R | 5′GAAGCTTTCAGTGCTGCTCGGCGTTCTGGT3′(HindIII) | |
| PAampG-I53S | 5′CCCAGCCAACTGGCGAAACCgctGGTATCCCGCGCCACGC3′ | Forward primer for I53S |
| PAampG-I53T | 5′CCCAGCCAACTGGCGAAACCggtGGTATCCCGCGCCACGC3′ | Forward primer for I53T |
| PAampG-53R | 5′GGTTTCGCCAGTTGGCTGGGGCTGGTGTACGCCTTCAAGT3′ | |
| PAampG-W90R | 5′AGCACCTGCGAGAACACCAGccgGGAACGGCGCCGGCCGA3′ | Forward primer for W90R |
| PAampG-W90S | 5′AGCACCTGCGAGAACACCAGcgaGGAACGGCGCCGGCCGA3′ | Forward primer for W90S |
| PAampG-90R | 5′CTGGTGTTCTCGCAGGTGCTGATCGCCCTGGGACTGCTCG3′ |
∗Underlined are restriction endonuclease sites; nucleotide sequence corresponding to the mutated amino acids is shown in lowercase.