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Schematic representation of the workflow and statistical comparisons.
GBM = glioblastoma; Reference RNA = Human Brain Reference RNA; 1 = Willcoxon Signed Rank Test between primary glioblastoma and corresponding recurrent glioblastoma using ΔCt; 2 = normalization to Human Brain reference RNA resulting in ΔΔCt; 3 and 4 = Mann-Whitney test between independent groups of samples using ΔΔCt (normalization of glioblastoma samples to Human Brain Reference RNA)
