. Author manuscript; available in PMC: 2018 Dec 10.

Published in final edited form as: Mol Imaging Biol. 2016 Oct;18(5):686–696. doi: 10.1007/s11307-016-0949-6

Table 1.

Spectral properties (position of maximum of excitation, λ_ex, and emission, λ_em), molecular weights and HPLC retention times of the fluorescent pHLIP^® variants are shown.

Spectral Properties
	AF546	AF647	AF750	Cy5.5	IR680	IR800	Dy680	DyP680
λ_ex, nm	556	650	753	630/673	672	778	680	680
λ_em, nm	572	670	778	720	702	797	707	707
Molecular Weights
WT	5146	5362	5462	4853	5140	5303	5084	5866
Var3	4256	4472	4572	3963	4250	4413	4194	4976
Var7	4100	4316	4416	3807	4094	4257	4038	4820
Var3-C	4313	-	-	4020	-	-	-	-
HPLC Retention Times
WT	29.2	24.8	25.3	29.4	25.5	25.0	26.2	24.9
Var3	27.6	23.3	23.6	28.4	24.3	23.4	25.0	23.7
Var7	25.7	21.6	22.0	26.9	22.8	21.6	23.9	22.0
Var3-C	27.5	-	-	29.8	-	-	-	-