Table 3.
Quantitative analytical results of anthocyanins and other phenolic metabolites in six-pink fruited blueberries and lowbush blueberry by LC-MS (µg/g DW)
| Phenolic compound | Pink-Lemonade (PLE) | Pink-Champagne (PCH) | Florida Rose (FLR) | US 2117 | US 2211 | US 2235 | Lowbush | |
|---|---|---|---|---|---|---|---|---|
| Anthocyanins | ||||||||
| 1 | Dp-3-gal* | 265.1±0.9 | 163.3±0.7 | 63.6±3.1 | 194.1±3.0 | 28.4±0.5 | 223.8±6.5 | 1017±3.2 |
| 2 | Dp-3-glc* | 52.3±2.5 | 182.2±3.7 | 48.6±2.9 | 28.5±0.2 | (BQL) | 465.9±12.8 | 3134±3.6 |
| 3 | Cyn-3-gal* | 133.5±2.9 | 60.8±1.1 | 44.6±1.2 | 67.6±0.2 | (BQL) | 75.7±5.3 | 858.2±20.4 |
| 4 | Dp-3-ara | 418.7±12.5 | 381.5±10.7 | 120.8±4.3 | 397.2±0.4 | 73.7±2.5 | 458.1±5.1 | 1266±31.5 |
| 5 | Cyn-3-glc* | (BQL) | 21.3±1.5 | (BQL) | (BQL) | ----- | 33.2±1.8 | 476.0±3.8 |
| 6 | Cyn-3-ara* | 129.7±6.7 | 74.9±0.1 | 36.7±2.3 | 65.9±0.6 | (BQL) | 978.0±3.5 | 648.8±13.0 |
| 7 | Pet-3-gal | (BQL) | (BQL) | (BQL) | 88.7±0.2 | (BQL) | 109.7±0.6 | 415.7±1.6 |
| 8 | Pet-3-glc* | (BQL) | 55.8±0.6 | (BQL) | (BQL) | … | 151.4±6.1 | 820.2±6.7 |
| 9 | Peo-3-gal | (BQL) | (BQL) | (BQL) | (BQL) | (BQL) | (BQL) | 136.1±1.6 |
| 10 | Pet-3-ara | (BQL) | (BQL) | (BQL) | 92.7±0.1 | (BQL) | 136.8±1.2 | 332.3±3.0 |
| 11 | Peo-3-glc* | ----- | (BQL) | (BQL) | ----- | ----- | 29.9±1.3 | 330.3±4.0 |
| 12 | Mv-3-gal* | (BQL) | 135.7±1.1 | 13.1± | 117.9±0.7 | 67.6±3.6 | 228.8±9.1 | 1030±21.4 |
| 13 | Mv-3-glc* | ----- | 54.7±0.0 | (BQL) | (BQL) | ----- | 179.6±1.1 | 1028±26.4 |
| 14 | Mv-3-ara | (BQL) | 208.7±1.6 | (BQL) | 142.0±0.5 | (BQL) | 252.5±0.9 | 895.1±4.1 |
| 15 | Dp-3-(ac-glc) | ----- | (BQL) | ----- | ----- | ----- | (BQL) | 772.5±9.8 |
| 16 | Pet-3-(ac-gal) | ----- | ----- | ----- | ----- | ----- | ----- | (BQL) |
| 17 | Peo-3-(ac-gal) | ----- | ----- | ----- | ----- | ----- | ----- | (BQL) |
| 18 | Cyn-3-(ac-glc) | ----- | (BQL) | ----- | ----- | ----- | (BQL) | 242.0±0.7 |
| 19 | Mv-3-(ac-gal) | ----- | (BQL) | ----- | (BQL) | (BQL) | ----- | 268.7±4.4 |
| 20 | Pet-3-(ac-glc) | ----- | (BQL) | ----- | ----- | ----- | (BQL) | 268.1±5.4 |
| 21 | Peo-3-(ac-glc) | ----- | (BQL) | ----- | ----- | ----- | .. | (BQL) |
| 22 | Mv-3-(ac-glc) | ----- | (BQL) | ----- | (BQL) | ----- | (BQL) | 589.6±23.6 |
| Total | 999.3 | 1339 | 327.4 | 1195 | 169.7 | 3323 | 14529 | |
| Flavan-3-ols | ||||||||
| 23 | Procyanidin B1* | 207.2±5.2 | 82.6±3.3 | 125.2±7.0 | 177.9±1.5 | 102.2±1.9 | 98.2±3.5 | 125.1±2.5 |
| 24 | Catechin* | 259.9±5.4 | 131.1±7.1 | 202.9±1.9 | 203.9±6.3 | 179.1±3.7 | 139.3±0.7 | 117.5±3.4 |
| 25 | Procyanidin B2* | 65.9±3.3 | 54.3±1.3 | 98.7±4.5 | 51.0±0.5 | 30.0±1.0 | 25.1±0.0 | 72.0±4.1 |
| 26 | Epi-catechin* | 55.6±2.1 | 53.5±4.8 | 109.9±1.2 | 57.1±1.5 | 40.3±0.1 | 26.0±0.9 | 74.6±0.5 |
| Total | 588.6 | 321.5 | 536.7 | 489.9 | 351.6 | 288.6 | 389.2 | |
| Flavonols | ||||||||
| 27 | Myrcetin-glc* | 121.8±4.8 | 71.9±0.5 | 65.2±4.1 | 172.2±1.7 | 29.1±2.5 | 26.5±0.2 | 130.5±4.5 |
| 28 | Quercetin-glc/gal* | 27.1±0.1 | 74.0±1.5 | 15.9±1.6 | 248.9±2.0 | 75.4±2.7 | 107.1±2.0 | 189.8±5.5 |
| 29 | Quercetin-ara* | 140.4±3.3 | ----- | 82.6±1.2 | 167.4±0.3 | 60.2±0.7 | 275.4±16.9 | 53.3±1.0 |
| 30 | Kaempferol-glc* | ----- | 441.0±28.0 | 271.6±7.4 | 79.5±0.4 | 475.3±38.1 | ---- | |
| 31 | Syringetin-glc* | ----- | 10.0±0.8 | 10.9±0.5 | 5.65±0.3 | 7.9±0.5 | 4.17±0.2 | 181.8±2.4 |
| 32 | Quercetin* | 15.4±0.2 | 5.6±0.0 | 7.52±0.3 | 13.1±0.1 | 7.8±0.5 | 5.72±0.3 | 12.2±0.4 |
| Total | 547.2 | 161.5 | 623.12 | 878.85 | 259.9 | 894.19 | 567.6 | |
| Phenolic acids | ||||||||
| 33 | Gallic* | 5.4±0.3 | 3.3±0.1 | 2.80±0.3 | 2.60±0.1 | 0.05±0.0 | 1.47±0.2 | 3.51±0.5 |
| 34 | 2.4 Hyroxybenzoic* | 66.3±9.8 | 36.7±2.1 | 62.6±4.1 | 50.7±6.0 | 52.2±1.9 | 39.4±1.5 | 72.7±9.7 |
| 35 | Caffeic* | 35.8±5.0 | ----- | 29.0±2.7 | ----- | 27.1±1.5 | ----- | 23.7±4.1 |
| 36 | Chlorogenic* | 3311±34.9 | 1492±24.8 | 2698±28.8 | 2586±41.6 | 2088±26.0 | 3020±32.8 | 4150±78.7 |
| 37 | Resveratrol* | 0.4±0.0 | 0.1±0.0 | 0.47±0.2 | 0.33±0.1 | 0.12±0.0 | 0.68±0.0 | 1.15±0.4 |
| Total phenolics | 5554 | 3354 | 4280 | 5203 | 2949 | 7568 | 19737 |
*
Reference standards available. Dp = delphinidin; Cyn = cyanidin; Pet = petunidin; Peo = peonidin; Mv = malvidin; gal = galactoside; glc = glucoside; ara = arabinoside; Anthocyanin compounds, with no reference standard available, were quantified as the closest anthocyanin compound having the same aglycone. BQL= detected but below the quantification limit