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. Author manuscript; available in PMC: 2018 Dec 10.
Published in final edited form as: Mol Immunol. 2008 Aug 19;46(1):27–36. doi: 10.1016/j.molimm.2008.06.030

Figure 7.

Figure 7

Allelic imbalance. Donor samples were screened for heterozygosity in the TLR3 gene using purified genomic DNA and a SNP detection assay (ABI). Heterozygous samples were then assayed for allelic imbalance using cDNA derived from total RNA obtained under the specified conditions. (A) Graphical representation of allelic ratios in heterozygous DC. cDNA samples were obtained by reverse transcription from day 6 DC total RNA, and allelic ratios (A:G or G:A) were determined using the real time PCR-based SNP assay. CB1-4: cord blood DC samples from separate donors; AD1-3: adult DC samples. (B, C) Influence of PI:C treatment on allelic imbalance. CB3 and CB5 samples were analyzed as in A, but total RNA samples were obtained at the indicated intervals in the presence or absence of PI:C. Bars depict the allelic ratios for each experimental time point. Note bias for allele G in CB3 and allele A in CB5.