Figure 3.

Knockdown of FAM3C did not influence cell proliferation, but did attenuate gastric cancer-cell migration in vitro.

Notes: (A) OD₄₅₀ values of control and FAM3C-knockdown cells at 24 hours, 48 hours, 72 hours, and 96 hours after the cells were seeded into 96-well plates showed no differences in AGS or MKN45 cells. (B, C) The number of formed colonies was not significantly different between control and FAM3C-knockdown cells. Images (B) and statistics (C) regarding the number of colonies. (D, E) Knockdown of FAM3C in AGS cells resulted in decreased wound-healing area 24 hours after scratching. Representative images (bar 200 µm) (D) and statistics (E) regarding the percentage of uncovered area. (F, G) Knockdown of FAM3C in AGS cells had reduced the number of migrated cells in the transwell assay at 24 hours after cell seeding. Representative images (bar 100 µm) (F) and statistics (G) regarding migrated cell numbers.

Abbreviation: NC, negative control.