FIG 3.

BAM complexes lacking BamC or BamE are hypersensitive to MAB1. (A and B) Bacterial growth curves for E. coli ΔwaaD ΔbamC (A) and E. coli ΔwaaD ΔbamE (B) with increasing concentrations of supplemented NaCl shown. (C) OmpT substrate cleavage assay for strains grown in medium alone. (D and E) Bacterial growth inhibition by MAB1 was measured by bacterial cell density (OD₆₀₀) in medium alone (D) or medium supplemented with 150 mM NaCl (E). (F and G) BamA protein levels were compared from mid-log cultures by Western blot (F) and FACS (G). Representative FACS traces are shown. (H) Membrane fluidity was increased in E. coli ΔwaaD ΔbamC and E. coli ΔwaaD ΔbamE. Data are expressed relative to E. coli ΔwaaD grown in medium alone. For all plotted experiments, means and SDs from biological triplicates are shown. ***, P < 0.001.