TABLE 1.
Plasmids and host strains
| Host straina | Plasmid | Protein | Mol wt (kDa) | Mutations |
|---|---|---|---|---|
| BL21.19 | pAL800 | properiOmpA | 18.5 |
ompA C290S and G244C residues, including signal sequence followed by residue 172 to the end, residue 325 |
| pAL942 | pRBPA248T | 31.0 | rbsB A248T, A188C, E246C | |
| pAL928 | pPhoA | 49.4 | phoA C169S, C179S, C287S, C337S, A369C, A427C | |
| pAL950 | pPhoE | 38.9 | phoE A249C, Q307C | |
| pAL831 | pLamB | 49.9 | lamB C22S, C38S, D329C, N386C | |
| pAL832 | proOmpAN176 | 21.2 | ompA T95C, I153C, truncated after residue 176 | |
| MM52 | pAL612 | proOmpA | 37.2 | ompA C290S, G244C |
| pAL725 | pGBP | 35.7 | mglB L244C, D287C | |
| pAL829 | pMBPY283D | 43.4 | malE Y283D, G289C, V347C |
a
BL21.19 (61) is a derivative of BL21(DE3) E. coli B F− dcm ompT hsdS(rB− mB−) gal λ(DE3) with secA13(Am), supF(Ts), trp(Am), zch::Tn10, clpA::KAN, and recA::CAT. MM52 is a derivative of MC4100 (56): F− (araD139)B/r secA51(ts) Δ(argF-lac)169 λ− flb-5301 Δ(fruK-yeiR)725(fruA25) relA1 rpsL150(strR) rbsR22 Δ(fimB-fimE)632(::IS1) deoC1.