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. 2018 Oct 10;2(12):1533–1549. doi: 10.1002/hep4.1266

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© 2018 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Mouse model of CF‐related cholangiopathy. C57BL/6J Cftr^‐/‐mice and Cftr^+/+littermates on an MCT diet were subjected to the following phenotypic analyses at the age of 3 months: body weight (A, left panel) and liver‐to‐body weight ratio (A, right panel); hematoxylin and eosin staining of liver tissue sections (B, a portal fibro‐inflammatory infiltrate is shown in inset); CK19 immunostaining of liver tissue sections (C, left panel) and morphometric analysis of CK19‐immunostained areas (C, right panel); sirius red staining of liver tissue sections (D, left panel) and count of mice according to the staging of fibrosis17 (F0: none; F1: portal fibrosis; F2: periportal fibrosis without bridging) (D, right panel). Scale bar: 100 µm; means ± SEM of at least 7 animals.