Figure 4.

OCA treatment reduces hepatic steatosis in 34‐week HFD‐fed Ldlr‐/‐.Leiden mice. Mice were treated with OCA (10 mg/kg/day) from t = 24 weeks to t = 34 weeks. (A) Representative images of hematoxylin and eosin–stained liver cross‐sections showing reduced hepatic steatosis in OCA‐treated mice. (B) Biochemically determined liver lipid content revealed a reduction in hepatic triglycerides and hepatic cholesteryl esters in OCA‐treated animals, whereas free cholesterol levels were not affected. Data are mean ± SD. *P < 0.05; ***P < 0.001 versus HFD at 34 weeks.