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. 2018 Sep 27;2(12):1583–1600. doi: 10.1002/hep4.1257

Figure 3.

Figure 3

Increased binding of E2F1 to the Wnt10a promoter in Phb1 KO livers. (A,B) Phb1 silencing in mouse primary hepatocytes induced Wnt7a and Wnt10a. Total RNA and protein were prepared and subjected to qPCR and western blot, respectively. Relative expression was represented as fold induction compared to NC; n = 3; *P < 0.05 versus NC. (C) Predicted E2F1 binding sites in the mouse Wnt10a (Accession ID, U61969.1) sequence as determined by ALGGEN PROMO Transcription Factor bindings prediction software. Transcription start site is shown as bold with GGC underlined. Underlined sequences represent primer sequences used for ChIP PCR assay. (D,E) E2F1 binding to the promoter was determined by ChIP assay of chromatin prepared from 3‐week‐old Phb1 KO and WT mice livers. ChIP assay was performed as described in the Materials and Methods. Relative target site occupancy is represented as fold over WT control. Results represent mean ± SEM from six WT and KO livers in duplicate experiments; *P < 0.05 versus WT.