Table 1.
Expression Pattern of WNT Ligands and PHB1 in Human HCC/CCA
| GSE Serial # | Description | Total Cases | Expression Ratio: HCC/Adjacent Nontumor Control | |||
|---|---|---|---|---|---|---|
| PHB1 | WNT9A | WNT10A | WNT7A | |||
| Down | Up | Up | Up | |||
| GSE76427 | Microarray expression data for tumor and adjacent nontumor tissues from patients with HCC | 114 | 57 (50%) | 67 (58%) 28 (49%) | 39 (34%) 27 (47%) | 29 (25%) 18 (32%) |
| GSE6764 | Early HCC versus normal liver tissue | 18 | 14 (78%) | 7 (39%) 5 (36%) | 2 (11%) 1 (7%) | 2 (11%) 1 (7%) |
| GSE14323 | RNA expression data for liver samples from subjects with HCC or normal liver | 38 | 18 (47%) | n/a | n/a | 13 (34%) 5 (28%) |
| GSE60502 | Gene expression profiling of HCC and adjacent nontumorous liver tissue | 18 | 11 (61%) | n/a | n/a | 9 (50%) 5 (45%) |
| GSE22058 | mRNAs from paired tumor and adjacent nontumor tissues from patients with HCC | 100 | 55 (55%) | 42 (42%) 22 (40%) | 13 (13%) 10 (40%) | 16 (16%) 5 (9%) |
| HCC tissues | mRNA levels in paired tumor/adjacent nontumor tissue and by qPCR assay | 18 pairs | 9 (50%) | 8 (44%) 4 (44%) | 8 (44%) 5 (56%) | 2 (11%) 1 (11%) |
| CCA tissues | mRNA levels in paired tumor/adjacent nontumor tissue and by qPCR assay | 7 pairs | 5 (71%) | 5 (71%) 3 (60%) | 5 (71%) 5 (100%) | 6 (86%) 4 (86%) |
Publicly available National Center for Biotechnology Information–GEO data sets as well as HCC/CCA tissues were analyzed to determine the pattern of PHB1 and WNT ligand expression. Upper row in each WNT category is the number (percentage) of patients with respective up‐regulated WNT ligand expression compared to the total number of cases. Values that are underlined are the number/percentage of patients with both WNT up‐regulation and PHB1 down‐regulation.
Abbreviation: GSE, gene series.