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TMPRSS2-ERG fusion targeting construct. A. The developed targeting construct fuses intron 2–3 of TMPRSS2 to intron 2–3 of ERG, which are approximately 3 Mbp apart. In addition, a eGFP-puro cassette is integrated in intron 3–4 of ERG. B. Used sgRNAs target intronic regions of both TMPRSS2 and ERG. C. Sequences targeted by used sgRNAs and the primers used to construct the sgRNA constructs following the protocol of Ran et al [27].
