Skip to main content
. Author manuscript; available in PMC: 2018 Dec 10.
Published in final edited form as: JSM Biotechnol Biomed Eng. 2017 Mar 28;4(1):1076.

Figure 2.

Figure 2

Transfection of mouse prostate organoids with the produced TMPRSS2-ERG targeting construct. A. Morphology of TMPRSS2-ERG organoids compared to WT organoids after clonal expansion of handpicked single organoids. B. Morphology of TMPRSS2-ERG organoid 10 passages after transfection. C. Ki67-staining on paraffin embedded organoids reveals increased proliferation in the fusion organoids compared to their wildtype counterparts. D. Conformation of TMRPSS2-ERG gene fusion on the DNA level. E. Sanger sequencing of the fusion-specific PCR on gDNA shown in D, reveals ligation of TMRPSS2 to ERG.