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. Author manuscript; available in PMC: 2018 Dec 10.
Published in final edited form as: JSM Biotechnol Biomed Eng. 2017 Mar 28;4(1):1076.

Figure 3.

Figure 3

ERG expression in TMPRSS2-ERG organoids is driven by AR signaling. A. Expression analysis of wildtype and three clonal TMPRSS2-ERG organoid lines. Expression of TMPRSS2 and ERG is calculated relative to β-actin. All expression values are calculated relative to wildtype organoids. B. Schematic overview of the effect of Nilutamide on ERG expression in TMRPSS2-ERG organoids. Due to the presence of an ARE in its protomer region, TMRPSS2 gene expression is driven by AR. As such, in TMPRSS2-ERG organoids, AR drives ERG overexpression. However, when Nilutamide is added to the medium, TMPRSS2 expression is prevented and ERG expression levels are restored to wildtype levels.