Fig. 3.

Evaluating the effectiveness of reversing formalin-induced crosslinking at GC regions (44–61% GC) of RPP30 amplicons. The data points represent the average values of the four samples in each extraction protocol with respect to their RPP30 amplicons. The dotted lines represent the best-fit line and the colours correspond to their respective extraction protocols. A) DNA extracted using phenol-chloroform and ethanol precipitation (PCE), Protocols I (blue) and V (orange), showed trend of decreasing averaged number of events with increasing GC-content of RPP30 amplicons. B) DNA extracted using column-based method from a commercial kit, Protocols II (yellow), III (red), and IV (grey), showed trend of slightly increasing averaged number of events with increasing GC-content of RPP30 amplicons. Please refer to Fig. 1 for detailed parameters of each extraction protocol (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).