Table 2.

qPCR Primers and Probes

Gene		Sequence	Source	IDT Reference No.
Ar	Fwd	5′ CTGCCTTGTTATCTAGCCTCA 3′	IDT	Mm.PT.47.17416675
	Rev	5′ ATACTGAATGACCGCCATCTG 3′
	Probe	5′ ACCACATGCACAAGCTGCCTCT 3′
Arg1	Fwd	5′ GAATGGAAGAGTCAGTGTGGT 3′	IDT	Mm.PT.58.8651372
	Rev	5′ AGTGTTGATGTCAGTGTGAGC 3′
	Probe	5′ ACAGTCTGGCAGTTGGAAGCATCT 3′
Bahcc1	Fwd	5′ GGTAGGAAGATGGATGCCAGA 3′	IDT	Mm.PT.58.12197037
	Rev	5′ CCACATCCTCTCCCTCTGA 3′
	Probe	5′ CACTCCCATGAAGCTCCTGCGTAC 3′
Egfl7	Fwd	5′ CAGCCTTACCTCACCACTTG 3′	IDT	Mm.PT.58.6782252
	Rev	5′ CAAGCATAGCGAGGCCTT 3′
	Probe	5′ CAGCACCTACCGAACCATCTACCG 3′
Epcam	Fwd	5′ GGCATTAAGCTCTCTGTGGAT 3′	IDT	Mm.PT.58.11851150
	Rev	5′ TGGTGTCATTAGCAGTCATCG 3′
	Probe	5′ ACCCATCTCCTTTATCTCCGACTTCTCA 3′
Esr1	Fwd	5′ CCTGTTTGCTCCTAACTTGCT 3′	IDT	Mm.PT.47.16003033
	Rev	5′ GAACCGACTTGACGTAGCC 3′
	Probe	5′ TGCCTTCCACACATTTACCTTGATTCCT 3′
Esr2	Fwd	5′ AAGTAGGAATGGTCAAGTGTGG 3′	IDT	Mm.PT.47.17681375
	Rev	5′ CTGGTTCTCTTGGCTTTGTTC 3′
	Probe	5′ AGTACGAAGACAGAGAAGTGCCAGC 3′
Fhl1	Fwd	5′ GTAGCGATTCTTATAATGCACCTC 3′	IDT	Mm.PT.58.9685318
	Rev	5′ GTCACTGCTGCCTGAAGT 3′
	Probe	5′ TTGACAAGTTCTGCGCCAACACC 3′
Gfap	Fwd	5′ CAACCTCCAGATCCGAGAAAC 3′	IDT	Mm.PT.51.11727348
	Rev	5′ GTCCTTGTGCTCCTGCTTC 3′
	Probe	5′ TCCTTAATGACCTCACCATCCCGC 3′
Gnrh1	Fwd	5′ GGGAAAGAGAAACACTGAACAC 3′	Custom
	Rev	5′ AGTACATTCGAAGTGCTGGG 3′
	Probe	5′ GATGGGCAAGGAGGTGGATCAAAT 3′
Hspa8	Fwd	5′ AAGAGCACAGGAAAGGAGAAC 3′	IDT	Mm.PT.58.33325534.gs
	Rev	5′ CTCAGCCTTGTACTTCTCAGC 3′
	Probe	5′ TTACTCAAGCGGCCCTTGTCATTGG 3′
Kiss1	Fwd	5′ CTGCTTCTCCTCTGTGTCG 3′	IDT	Mm.PT.45.16269514
	Rev	5′ TTCCCAGGCATTAACGAGTTC 3′
	Probe	5′ CGGACTGCTGGCCTGTGGAT 3′
Kiss1r	Fwd	5′ CTCACTGCATGTCCTACAGC 3′	IDT	Mm.PT.49a.16255718.g
	Rev	5′ GCCTGTCTGAAGTGTGAACC 3′
	Probe	5′ TCAATCCGCTGCTCTATGCCTTCC 3′
Npffr1	Fwd	5′ GACAATGCCACATGCAAGATG 3′	IDT	Mm.PT.49a.12363343
	Rev	5′ GTCAGCTTCTCACGGAAAGG 3′
	Probe	5′ TCTGCGTCGGTTTTCACACTGGT 3′
Otx1	Fwd	5′ GAAGTCCTTCAAGCTGTTGC 3′	IDT	Mm.PT.58.42606516
	Rev	5′ CTAAACAACCGAGCAAGACAAG 3′
	Probe	5′ AGAGGTAGATGGTGAAAAGCCGCG 3′
Otx2	Fwd	5′ TTGTTCTGACCTCCATTCTGA 3′	IDT	Mm.PT.58.13383028
	Rev	3′ AAATCAACTTGCCAGAATCCAG 3′
	Probe	5′ ATCGAAGAGCTAAGTGCCGCCAA 3′
Oxt	Fwd	3′ CTTGGCTTACTGGCTCTGAC 5′	IDT	Mm.PT.58.29698679.g
	Rev	3′ CCGAAGCAGCGTCCTTT 5′
	Probe	5′ TGTGCTGGACCTGGATATGCGC 3′
Pgr	Fwd	5′ CGCCATACCTTAACTACCTGAG 3′	IDT	Mm.PT.47.10254276
	Rev	5′ CCATAGTGACAGCCAGATGC 3′
	Probe	5′ AGATTCAGAAGCCAGCCAGAGCC 3′
Ppia	Fwd	5′ CAAACACAAACGGTTCCCAG 3′	IDT	Mm.PT.39a.2.gs
	Rev	5′ TTCACCTTCCCAAAGACCAC 3′
	Probe	5′ TGCTTGCCATCCAGCCATTCAG 3′
Rab25	Fwd	5′ TGTCGTGGCTGAACTCATTG 3′	IDT	Mm.PT.58.42268495
	Rev	5′ CGTCTTCACTCTCCATGCTG 3′
	Probe	5′ CACGCCTGACTCGCCGATCA 3′
Rai1	Fwd	5′ CAAGAGAAAAGGGAGACTGAGAG 3′	IDT	Mm.PT.58.9078821
	Rev	5′ TCTCGAAAAGACTGCATGACT 3′
	Probe	5′ AGGAGCCCGCAGATAACCAGC 3′
Six1	Fwd	5′ GAGAGAGTTGATTCTGCTTGTTG 3′	IDT	Mm.PT.58.13903884
	Rev	5′ GGTCAGCAACTGGTTTAAGAAC 3′
	Probe	5′ CGAGGCCAAGGAAAGGGAGAACA 3′
Six6	Fwd	5′ GGTCCCTCACCCTCCGA 3′	IDT	Mm.PT.58.43583201
	Rev	5′ CAAAAACCGCAGACAAAGAGAC 3′
	Probe	5′ TGCAGCCAAAAACAGACTCCAGC 3′
Tmprss6	Fwd	5′ CCCAAAGGAGTAGACAGAACTAGA 3′	IDT	Mm.PT.58.42367147
	Rev	5′ CTATTGCTTTCCGCAGTGAATC 3′
	Probe	5′ CCAAAGCCCAGAAGATGCTCCAAGA 3′
Tnrc18	Fwd	5′ AAGAACTGCTCTACCTGCAAC 3′	IDT	Mm.PT.58.11343509
	Rev	5′ ATCTTCTCCTCCATCTCCGT 3′
	Probe	5′ CTCTTGTGCTTTCAACCGCTCCAC 3′’
Tstd1	Fwd	5′ GCGATTTCCAATTCAGACACAG 3′	IDT	Mm.PT.58.10697454.g
	Rev	5′ CGTTCACTCGTAGCCTCAG 3′
	Probe	5′ TGTTGAGTGCCCCAGGAATGGTAC 3′

Primer probe sets that may amplify genomic DNA are indicated in bold.

Abbreviations: Fwd, forward; IDT, Integrated DNA Technologies; Rev, reverse.