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. Author manuscript; available in PMC: 2018 Dec 10.
Published in final edited form as: Differentiation. 2018 May 17;102:1–9. doi: 10.1016/j.diff.2018.05.002

Fig. 4.

Fig. 4.

Ex vivo imaging of Y-suture formation in tdT labelled mouse lenses. (A-D, I-L) Anteriorpole view of the wild-type-tdT (A, I), Epha2-null-tdT (B, J), Efna5-null-tdT (C, K), and Epha2/Efna5-null-tdT (D, L) lenses showing grossly disturbed formation (B-D, J-L) of the upright Y-suture pattern (arrows) found in wild-type (A, I) at P7 (A-D) and P30 (I-L). (E-H, M-P) Posterior-pole view of the wild-type-tdT (E, M), Epha2-null-tdT (F, N), Efna5-null-tdT (G, O), and Epha2/Efna5-null-tdT (H, P) lenses showing variably and progressively disturbed formation (FH, N-P) of the inverted Y-suture pattern (arrows) found in wild-type (E, M) at P7 (E-H) and P30 (M-P). Image depth from lens surface: 100 – 150 μm (A-D, E, G, I-L, M), 100 – 50 μm (F, H, N). Scale bar: 100 μm. (n = 80, 76, 24, and 8 for wild-type, Epha2-null, Efna5-null, and Epha2/Efna5-null lenses, respectively, with representative images shown).