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Steady-state levels of SPP1 mRNA in endometria determined by slot blot hybridization analysis. SPP1 expression was not different between nongravid (black bars) and gravid (white bars) uterine horns within the respective days of pregnancy (P > 0.10). However, SPP1 mRNA levels significantly increased between gestational Days 40 and 80 in the gravid horn (P < 0.01) as well as between Days 80 and 120 (P < 0.01) in the nongravid horn.
