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. 2018 May 24;9(6):523–539. doi: 10.1080/19490976.2018.1458180

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Figure 1. — Conversion of [24-¹⁴C]-cholic acid by putative bile acid 7α-dehydroxylating strains. First section of autoradiograph of TLC plate includes cholic acid (CA), deoxycholic acid (DCA), and allodeoxycholic acid (ADCA) TLC standards. The second autoradiographic section contains [24-¹⁴C]-cholic acid metabolites from several bile acid metabolizing strains, including strain C592. Each isolate was grown anaerobically (N₂) in Balch tubes in the presence of 25 µM cholic acid along with 1 µCi [24-¹⁴C]-cholic acid. Cultures were extracted with ethyl acetate and separated on TLC as described in the Materials and Methods.