Table 1.

Activation and inactivation parameters for ASD mutations A749G and G407R.

	activation				inactivation
	Vrev [mV]	Slope [mV]	V0.5 [mV]	n	V0.5 [mV]	Slope [mV]	Remaining current [%]	n
WTS	64.9 ± 1.04	7.71 ± 0.11	−9.49 ± 0.42	66	−31.7 ± 0.88	4.31 ± 0.12	11.5 ± 2.18	25
AGS	54.7 ± 1.46***	6.61 ± 0.11***	−18.3 ± 1.34***	13	−48.6 ± 1.58***	4.27 ± 0.16	6.01 ± 1.50	9
GRS	61.6 ± 1.77	7.68 ± 0.28	−15.3 ± 2.20***	14	nd
WTL	67.7 ± 1.14	8.92 ± 0.20	−2.55 ± 1.05	29	25.7 ± 2.08	5.56 ± 0.23	14.4 ± 3.12	18
AGL	60.3 ± 0.83###	7.14 ± 0.20###	−12.3 ± 0.87###	27	−41.1 ± 1.07###	5.82 ± 0.18	9.43 ± 1.49	14
GRL	54.7 ± 2.32###	7.90 ± 0.30	−6.58 ± 1.41	13	nd

All values are presented as mean ± S.E.M. Number of independent transfections >3. Parameters were obtained after fitting normalized (I/I_max) I-V relationships or normalized (I/I_max) steady-state inactivation curves, as described in Methods. Statistical analysis was performed using one-way ANOVA and Bonferroni post-hoc test (activation) or unpaired Student’s t-test (steady-state inactivation). Significance **^{, ##} p < 0.01, ***^{, ###} p < 0.001 in comparison to WT_S (*) or WT_L (^#) (statistical analysis has been performed separately for long and short splice variants). n, number of experiments; nd, not determined; V_0.5, half maximal activation/inactivation voltage; V_rev, reversal potential; WT, wild-type. Note small differences among WT recordings within different sets of experiments (Tables 1, 3 and 5) because WT control experiments were always carried out in parallel with mutated channels. Reference data, which were already published previously, are indicated in italics (taken from reference 10).