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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Exp Neurol. 2018 Oct 19;311:247–256. doi: 10.1016/j.expneurol.2018.10.010

Figure 1.

Figure 1.

Isolation and RNA-seq profiling of wild-type (WT) Scn1a+/− mice on 129 and F1strains at P14 and P24. A) Schematic showing collection and pooling of samples comprising the nine experimental groups. Dissected hippocampi from 4 animals (2 male, 2 female) were pooled prior to RNA isolation. Following RNA isolation, 3–4 samples with RIN>7.6 were combined to generate superpools (n=12–16 mice/superpool). For RNA-seq analysis 2–3 superpool biological replicates were used for each group. The number of replicates is shown in the lower left corner of the box representing the experimental groups. P24 F1.Scn1a+/− samples were further subdivided into two groups: without seizures (no shading), or with ≥3 seizures (yellow shading) within 24 hours of sample collection. B&C) Sample-to-sample distances visualized by principal component analysis for experimental groups with exclusion (B) or inclusion (C) of F1.Scn1a+/− mice with seizures.