Figure 2:

Adoptively transferred T_H17 cells accumulate in the hippocampus after the learned-helplessness paradigm. (A) CD45.2 wild-type recipient mice received donor CD45.1⁺CD4⁺ T cells or CD45.1⁺ T_H17 cells, and 48 h later were subjected to the learned-helplessness paradigm. 86% of the CD4⁺T cells transferred in the T_H17 cells preparation were T_H17 cells, whereas less than 1% T_H17 cells were detected in the CD4 preparation, and 99.3% of the injected CD4 cells were CD45.1. (B) CD4⁺ CD45.1⁺ (donor cells) or CD4⁺CD45.2⁺ (recipient cells) present in the hippocampus of mice adoptively transferred with CD4 cells or T_H17 cells were analyzed by flow cytometry after gating on CD4⁺ cells (C) CD45.1⁺CD4⁺ (donor cells) or CD45.2⁺IL-17A⁺CD4⁺ (recipient cells) present in the hippocampus of mice adoptively transferred with CD4 cells or T_H17 cells were analyzed by flow cytometry after gating on CD45⁺CD4⁺ cells. Each symbol represents an individual mouse. Data represent mean±SEM, one-way ANOVA, F(3, 16) = 9.522, Bonferroni post-hoc test *p<0.05 (n=5). (D) CD45.1⁺IL-17A⁺CD4⁺ (donor cells) or CD45.2⁺IL-17A⁺CD4⁺ (recipient cells) present in the hippocampus of mice adoptively transferred with CD4 cells or T_H17 cells were analyzed by flow cytometry after gating on CD45⁺IL-17A⁺CD4⁺ cells. Each symbol represents an individual mouse. Data represent mean±SEM, one-way ANOVA, F(3, 14)=9,795, Bonferroni post-hoc test *p<0.05 (n=4-5).