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. 2018 Nov 2;8(1):e1512458. doi: 10.1080/2162402X.2018.1512458

Figure 1.

Figure 1.

Comprehensive flow cytometric analyses of lymphocytic subsets in gastro esophageal adenocarcinoma. Single cell suspensions of primary tumor samples (n = 28), tumors of patients following neoadjuvant treatment (n = 19) or normal mucosa (n = 23) were analyzed for the percentage of CD45+ lymphocytes by flow cytometry (A). Lymphocytic subsets in PBMC of healthy controls (PBMC HC, n = 20), PBMC of untreated tumor patients (PBMC AC, n = 46), tumor samples (n = 28) and tumor-draining lymph nodes (TDLN, n = 23) were analyzed for the percentages of total B cells (B), CD86+ activated B cells (C), IgD−CD27+ memory B cells (D), CD3+PD-1+ activated T cells (E) and CCR7−CD45RA− effector memory T cells (F). Additional plots compare B cell subsets in low (UICC I and II) and high (UICC III and IV) tumor stages (B-F). Graphs show mean values ± SEM, p = ANOVA, *p < 0.05.