Figure 4.

Analysis of the tumor antigen-specific B cell response in gastro-esophageal adenocarcinoma. LUMINEX^TM analyses of 34 TAAs were performed in serum samples of untreated gastro-esophageal adenocarcinoma patients (n = 34), serum samples following neoadjuvant chemoradiotherapy (n = 7) and healthy controls (n = 5) was analyzed by LUMINEX^TM (A). Individual MFIs for CTAG1A and MAGEA4 (B). Summary of antibody responses detected in serum samples of tumor patients and healthy controls (C) Exemplary flow cytometry analyses of b cells specific for NY-ESO-1 (CTAG1A) in PBMC and TDLN of a gastro-esophageal adenocarcinoma patient using biotinylated NY-ESO-1 and a streptavidin tetramer (D). Heatmap and bar graphs of LUMINEX^TM data show mean MFIs of duplicates, p = Chi² test.