Figure 1.
Characterization of the novel HPV16 E6/E7-expressing A2.DR1 tumor cell line, PAP-A2. (A) PAP-A2 cells were lysed and analyzed for E6 (left) and E7 (right) expression by Western blot. CaSki: HPV16+ cervical cancer cells, positive control; 2277NS: untransduced parental cell line of PAP-A2, negative control; PAP-A2: cell line lysate; PAP-A2 tumor: lysate generated from a PAP-A2 tumor having grown in an A2.DR1 mouse. The Western blots shown are representative of three Western blots. (B) Tumor growth curves of PAP-A2 cell injection number titrations in A2.DR1 mice. Mean ± SD are shown. (C) Mouse survival curves for the experiment shown in B. n = 3 (5x106) or n = 5 (other groups) mice per group.