Figure 5.

IL-33 promotes activation of peritoneal macrophages.

Mice were challenged with intraperitoneal ID8-Defb29/Vegf-a tumors and treated at days 7,14 and 21 with intraperitoneal IL-33 or PBS. Two days later we performed a peritoneal wash and performed peritoneal cell staining for flow cytometry and flow cytometry associated cell sorting of CD11b⁺F4/80⁺ peritoneal macrophages. (A) Quantitative real time PCR showing relative quantification of Ym1, (B) IL-13, (C) ST2 and (D) IL-10 from IL-33 treated mice relative to macrophages from PBS treated mice. Mean fluorescence intensity of macrophages stained for (E) CD40, (F) CD80 and (G) CD86 from IL-33 or PBS treated mice. (triplicates, pooled from 5 mice per group, 2 independent experiments, 2 independent experiments). t-test. *p < 0.05 ***p < 0.001