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. 2018 Dec 10;13(12):e0204538. doi: 10.1371/journal.pone.0204538

Fig 3. KCl regulates HDAC3:NCOR2 catalytic activity.

Fig 3

HDAC3:NCOR2 activity and its dependency on KCl concentration was determined by constructing Michaelis-Menten plots (a representative plot is shown in panel A at 5mM [KCl]) at varying concentrations of KCl (0.05 mM-50 mM), Fluor de Lys SIRT1 substrate (1.56 μM-100 μM) and 0.1 μM HDAC3:NCOR2 in 25 mM Tris pH 8.0 with 500 μM EDTA. Initial velocities were determined from time course data based on changes in fluorescence. Catalytic parameters were determined and are summarized in Table 1. Eq 1 was used to fit the bell-shaped dependency of HDAC3:NCOR2 activity with varying [KCl] (panel B) yielding K1/2,act and K1/2,inhib values.