Figure 3.

Tcf21 is expressed in a subset of the CM and stromal mesenchyme. (A) Immunostaining of the kidney from Tcf21LacZ/+; FoxD1Cre; (ROSA26)mT/mG triple transgenic mice. Tcf21 LacZ expression by β-galactosidase staining (red) delineating normal expression of Tcf21 at E18.5. Derivatives of FoxD1-positive stromal mesenchyme was visualized by GFP using FoxD1-Cre;(ROSA26)mT/mG reporter mice (green). CM is represented by Six2 immunostaining (blue). Bar, 100 μm. (B) Schematic depiction of Tcf21 deletion in the mouse models utilized. White areas represent Tcf21-deleted cell lineages. (i) Wild-type; (ii) Tcf21 null kidney, Tcf21 is deleted in germline cells and hence from the entire MM and its derivatives; (iii) CapTcf21 kidney, Tcf21 is selectively deleted from the CM under Six2-eGFPCre driver; (iv) StrTcf21 kidney, Tcf21 is selectively deleted from the stromal mesenchyme under Foxd1-eGFPCre driver.