Figure 5.

ID-8 (1 µM) modulates cell cycle response, upregulating proproliferative genes. (A) Principal component analysis shows distinct distribution of the cells treated for 48 hours with ID-8 and harmine in comparison with undamaged cells (control), no treatment after damage, or treated with EGF (full medium) after hypoxia-induced damage. (B) Hierarchical clustering of genes differentially expressed between cells treated for 48 hours with ID-8 or harmine and untreated cells. (C) Venn diagrams showing the number of differentially upregulated and downregulated genes in cells treated for 48 hours with ID-8, harmine, and untreated cells; FC>1.5. (D) Gene ontology of the top modulated pathways in cells treated for 48 hours with ID-8 or harmine compared with untreated cells.