Fig 4. Mutations at nucleosomes parsed by chromatin state and histone modification.

Mutations enrichments (solid blue lines) at nucleosomes across multiple chromatin states (A-G) and with different pre-existing histone modifications (H-M) show the same ~10 bp periodicity. Overall mutation enrichment varied widely across all chromatin states (p-value = 0.0014 by Kruskal-Wallis) and all histone modification-sorted nucleosomes (p-value = 2.55x10-6 by Kruskal-Wallis) with transcription elongation (A) and heterochromatin (G) and H3K36me3 (K) and H3K27me3 (J) being some the most different pairs (p-values = 0.0357 and 0.002, respectively by Dunn’s Test).