Figure 5.

Anti-PD-1 treatment and irradiation induces an abscopal effect in B16 tumors. B16 tumors were established intracranially and subcutaneously in the flank similar to those using D4M cells. Brain (A.) and flank (B.) tumor growth was followed by bioluminescence imaging and graphs represent percent in growth. C. D. At the conclusion of the experiment, tumors were harvested and dissociated by collagenase/hyaluronidase digestion followed by staining for CD8⁺ T cell markers. Cells were then read-out and quantified by flow cytometry. *p < 0.5 and **p < 0.01 for the indicated comparisons between treatment groups. For the tumor growth graph. *p < 0.05 for the combination group vs. others at the final time point using ANOVA analysis.