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. Author manuscript; available in PMC: 2018 Dec 10.
Published in final edited form as: Cell Rep. 2018 Nov 13;25(7):1729–1740.e6. doi: 10.1016/j.celrep.2018.10.052

Figure 3. usRC Protects RC-Proximal Genes from Eβ-Dependent Transcriptional Activation.

Figure 3.

(A) Scale schematic of the Tcrb locus indicating gene segments assayed.

(B–D) Transcription of Vβ gene segments and RC-proximal trypsinogen genes were analyzed by RT-qPCR. (B) Effects of usRC and Eβ. (C) Dissection of usRC. (D) Effects of compound usRC deletions. Vβ primers only detect transcripts originating from unrearranged gene segments on the experimental allele. Expression values are normalized to Actb, with Actb expression set to 4 on a log10 scale. The data represent the mean and SE of two to eight independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, by two-way ANOVA with Holm-Sidak’s multiple-comparisons test. Number sign, not detectable. The same data for wild-type and ΔusRC were plotted in all three panels.