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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Methods Enzymol. 2018;606:389–420. doi: 10.1016/bs.mie.2018.04.002

Fig. 5.

Fig. 5

A representative continuous wave X-band EPR spectrum of K. pneumoniae PqqE samples in various conditions. The signals are as follows, from top to bottom: dithionite (DTH)-reduced PqqE with a 10-fold excess of SAM (with and without glycerol), DTH-reduced PqqE (with and without glycerol), and as-isolated PqqE with glycerol. Adapted from Wecksler, S. R., Stoll, S., Tran, H., Magnusson, O. T., Wu, S. P., King, D., et al. (2009). Pyrroloquinoline quinone biogenesis: demonstration that PqqE from Klebsiella pneumoniae is a radical S-adenosyl-L-methionine enzyme. Biochemistry, 48(42), 10151–10161. Copyright © 2009 American Chemical Society.