Figure 1.

Acamprosate treatment in ENT1^−/− mice during two-bottle choice alcohol administration. A. Schematic representation of the experimental paradigm. Mice were given 18 days to acclimate to a 10% (v/v) ethanol concentration. Then mice were injected with either saline or acamprosate twice per day (2 times x 200mg/kg, i.p.) for the next 5 days during the two-bottle choice self-administration (n = 9 ~10). B. There was no difference in the blood acamprosate levels between genotypes. C. Effect of Acamprosate treatment in alcohol consumption. Alcohol consumption (g/kg/day) is reduced in both genotypes with acamprosate treatment. D. Ethanol preference (%) is significantly reduced in in ENT1 ^–/– mice. E. Water drinking is similar between genotypes during acamprosate treatment. All data are presented as mean ± SEM.