(A) Predicted protein kinases that phosphorylate threonine residues in
the MBP 80-102 region. Asterisk, CDK5 (PhosphoNET, www.kinexus.ca). (B) Predicted CDK5 phosphorylation sites (red
arrows) in human (NP_001020261, MBP isoform 3), rat (NP_001020463, MBP isoform
2) and mouse (NP_001020426, MBP isoform 3) MBP sequences. Consensus sequences
are indicated by yellow boxes. Predictions were made using NetPhos tool
(http://www.cbs.dtu.dk/services/NetPhos-2.0). Only sites with
high confidence scores (>0.5) are shown. MBP84-104 peptide sequence is
shown as a blue bar. Conserved residues are shaded black. (C) CDK5/p25 kinase
complexes phosphorylate the MBP84-104-WT, -H89G, -pT96, -pT99 peptides but not
the MBP84-104-SCR peptide. Bars correspond to the mean normalized cpm (n=3; **,
p≤0.005).