Skip to main content
. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: FEBS J. 2018 Aug 27;285(18):3485–3502. doi: 10.1111/febs.14623

Fig. 5. Predicted phosphorylation sites of MBP conserved central region.

Fig. 5.

(A) Predicted protein kinases that phosphorylate threonine residues in the MBP 80-102 region. Asterisk, CDK5 (PhosphoNET, www.kinexus.ca). (B) Predicted CDK5 phosphorylation sites (red arrows) in human (NP_001020261, MBP isoform 3), rat (NP_001020463, MBP isoform 2) and mouse (NP_001020426, MBP isoform 3) MBP sequences. Consensus sequences are indicated by yellow boxes. Predictions were made using NetPhos tool (http://www.cbs.dtu.dk/services/NetPhos-2.0). Only sites with high confidence scores (>0.5) are shown. MBP84-104 peptide sequence is shown as a blue bar. Conserved residues are shaded black. (C) CDK5/p25 kinase complexes phosphorylate the MBP84-104-WT, -H89G, -pT96, -pT99 peptides but not the MBP84-104-SCR peptide. Bars correspond to the mean normalized cpm (n=3; **, p≤0.005).