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. Author manuscript; available in PMC: 2018 Dec 10.
Published in final edited form as: Dev Biol. 2016 Apr 16;426(2):165–175. doi: 10.1016/j.ydbio.2016.03.021

Figure 5. Expression of Wnt/β-catenin target genes is reduced by dkk-injection.

Figure 5.

(A,C,E) Normalized counts from RNAseq data comparing uninjected control (UC) and dkk-injected embryos for selected known (A) and candidate (C,E) target genes at stage 11.5 (mid-gastrula). (B,D,F) In situ hybridization showing expression pattern of known (B) and candidate (D, F) target genes. Embryos were injected in the right (asterisk) 2 blastomeres at the 4-cell stage with a total of 100 pg dkk. Blue box indicates that embryo was cleared in either benzyl benzoate:benzoic acid (BB:BA) or benzyl benzoate alone (BB; to reduce transparency and visualize the archenteron). Blastopore view with dorsal up, except pnhd is dorsal view with anterior up.