Table 2.
Effect of different temperatures and pH on gelatinolytic activity of B. subtilis LFB-FIOCRUZ 1266 mutants and wild-type strains
| T (°C) | pH | WT | D2 | D7 | D8 | D11 | E2 | E4 | E5 | E12 | E13 | G4 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 28 | 8.0 | 880.7 | 948.9 | 659.1 | 630.7 | 926.1 | 875.0 | 1210.3 | 1068.2 | 755.7 | 522.7 | 358.0 |
| 30 | 6.0 | 39.8 | 233.0 | 125.0 | 181.8 | 34.1 | 62.5 | 261.4 | 227.3 | 278.4 | 178.0 | 200.8 |
| 30 | 10.0 | 284.1 | 153.4 | 403.4 | 409.1 | 272.7 | 352.3 | 329.6 | 210.2 | 153.4 | 102.3 | 306.8 |
| 35 | 5.2 | 352.3 | 340.9 | 242.4 | 284.1 | 272.7 | 619.3 | 45.5 | 89.0 | 187.5 | 147.7 | 193.2 |
| 35 | 8.0 | 123.1 | 535.4 | 372.5 | 352.3 | 520.8 | 433.7 | 524.6 | 275.3 | 780.3 | 153.4 | 356.1 |
| 35 | 10.8 | 352.3 | 227.3 | 312.5 | 85.2 | 335.2 | 335.2 | 380.7 | 431.8 | 181.8 | 354.2 | 272.7 |
| 40 | 6.0 | 176.1 | 340.9 | 193.2 | 431.8 | 409.1 | 375.0 | 403.4 | 380.7 | 250.0 | 113.6 | 121.2 |
| 40 | 10.0 | 176.1 | 142.1 | 136.4 | 128.8 | 204.6 | 244.3 | 204.6 | 66.3 | 56.8 | 108.0 | 157.2 |
| 42 | 8.0 | 386.4 | 409.1 | 136.4 | 301.1 | 448.9 | 448.9 | 511.4 | 352.3 | 267.1 | 253.8 | 210.2 |