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. 2018 Nov 29;131(23):jcs222042. doi: 10.1242/jcs.222042

Fig. 3.

Fig. 3.

Tpm3.5 is localized in small puncta along the fiber cell membrane. Immunostaining of single fiber cells from 6-week-old Tpm3/Δexon9d+/+ and Tpm3/Δexon9d−/− lenses for Tpm3.5 (green) and F-actin (red). Images are single optical sections through the cell cytoplasm. (A) In the mature Tpm3/Δexon9d+/+ fiber cell, Tpm3.5 is enriched in puncta along the cell membrane, colocalizing with F-actin. There is an obvious decrease in Tpm3.5 staining in the mature Tpm3/Δexon9d−/− lens fiber. The mutant mature fiber has large paddle domains decorated by small protrusions similar to those in the control lens fiber. (B) An enlargement of a paddle region from the control mature fiber reveals Tpm3.5 puncta along the cell membrane at the tips of and near the base of small protrusions (arrows). In the mutant lens fiber, Tpm3.5 staining is drastically reduced at the cell membrane and remaining small puncta containing Tpm3.5 are mostly at the base of small protrusions (open arrowheads). Scale bars: 5 µm (A); 2 μm (B).