Skip to main content
. 2018 Nov 29;131(23):jcs222042. doi: 10.1242/jcs.222042

Fig. 7.

Fig. 7.

Reduced levels of Tpm3.5 affects levels of actin and select actin-associated proteins, Tmod1, N-cadherin, ezrin and Arp3. Western blots of indicated proteins from 6-week-old Tpm3/Δexon9d+/+ and Tpm3/Δexon9d−/− whole lenses. All protein levels were normalized to total protein level (Ponceau S staining). Membrane skeleton components, Tmod1 and actin, are decreased in Tpm3/Δexon9d−/− lenses, while β2-spectrin levels remained unchanged. Actin-associated proteins that link F-actin to the cell membrane, N-cadherin and ezrin, have decreased levels in mutant lenses, while the level of ankyrin-B is unaffected. Arp3, a nucleating protein for branched F-actin networks, is decreased in mutant lenses, but levels of F-actin cross-linking proteins, α-actinin (Actn1) and fimbrin, as well as NMIIA and NMIIB are unchanged. Decreased Tpm3.5 does not affect beaded intermediate filament proteins, CP49 and filensin, levels. Plots reflect the mean±s.d. of n=3 independent protein samples per genotype. *P<0.05; **P<0.01.