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. 2018 Dec 10;4:40. doi: 10.1038/s41523-018-0095-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Detection of B7-H4 protein expression by using immunofluorescence (QIF) in breast cancer. a Representative fluorescence image showing staining for B7-H4 (red channel), cytokeratin (green channel), and nuclei (4′,6-diamidino-2-phenylindole; DAPI) (blue channel). b, c Magnified areas of a. b Target protein, B7-H4 (red channel), is highly expressed within stroma. c Target protein, B7-H4 (red channel), and is highly expressed within tumor. Bar = 100 μm