Table 3.

Association of B7-H4, PD-L1 expression by QIF and TILs count in tumor and stromal components of primary breast cancer

Yale cohorts
	PDL1		N a	p-Valueb
	Negative	Positive
Tumor B7H4
Negative	267 (47%)	37 (7%)	304	<0.0001
Positive	251 (45%)	6 (1%)	257
	518	43	561
Stromal B7H4
Negative	292 (60%)	37 (7%)	329	0.0018
Positive	156 (32%)	5 (1%)	161
	448	42	490

	TIL		N	p-Value
	Low	High
Tumor B7H4
Negative	147 (36%)	34 (8%)	181	<0.0001
Positive	216 (53%)	10 (3%)	226
	363	44	407
Stromal B7H4
Negative	242 (59%)	39 (10%)	281	0.003
Positive	121 (30%)	5 (1%)	126
	363	44	407
Tumor PD-L1
Negative	359 (88%)	7 (2%)	366	<0.0001
Positive	4 (1%)	37 (9%)	41
	363	44	407
Stromal B7H4
Negative	331 (81%)	25 (6%)	356	<0.0001
Positive	32 (8%)	19 (5%)	51
	363	44	407

UM cohort
	PDL1		N a	p-Valueb
	Negative	Positive
Tumor B7H4
Negative	175 (41%)	59 (14%)	234	<0.0001
Positive	173 (41%)	17 (%4)	190
	348	76	424
Stromal B7H4
Negative	265 (63%)	45 (11%)	310	0.82
Positive	95 (23%)	15 (3%)	110
	360	60	420

	TIL		N	p-Value
	Low	High
Tumor B7H4
Negative	174 (41%)	52 (12%)	226	<0.0001
Positive	188 (45%)	7 (2%)	195
	362	59	421
Stromal B7H4
Negative	249 (59%)	57 (13%)	306	<0.0001
Positive	113 (27%)	3 (1%)	116
	362	60	422
Tumor PD-L1
Negative	323 (78%)	21 (5%)	344	<0.0001
Positive	34 (8%)	38 (9%)	72
	357	59	416
Stromal B7H4
Negative	317 (77%)	35 (8%)	352	<0.0001
Positive	37 (9%)	24 (6%)	61
	354	59	413

^aOnly cases which had staining for both markers were included to analysis

^bQIF signals between groups presented in frequency tables evaluated by Chi-Square test. Two sided p-values are considered statistically significant if <0.05 and are indicated in bold