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. 2018 Dec 10;8:17759. doi: 10.1038/s41598-018-36009-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Method of estimating mean diffusion radius using fluorescence thresholding to detect maximum and minimum detectable spread of RD. (a) Representative image of patched skin, pseudocoloured to represent RD. (b) Fluorescence threshold at 5%. (c) Fluorescence threshold 95%. Three to four puncture holes were selected for analysis; shown here are seven holes for demonstration purposes. Overlay graphics generated from Matlab code directly.