Fig. 4.

Dasatinib induces an epithelial- and luminal-like phenotype and differentiation of chemo-resistant cells. a Cell morphology (20× magnification) of SUM159-P cells treated with DMSO or dasatinib (1 uM) for 4 days. b, c SUM159-P cells were treated with DMSO or dasatinib for 2 days. Then the mRNA and protein levels of indicated genes were subjected to real-time qPCR and immunoblotting analysis. d MUC1 mRNA expression was analysed in SUM159-P cells treated with or without dasatinib. e, f Percentage of MUC1+ population was analysed by flow cytometry in SUM159-P cells treated with dasatinib. g SUM159-P cells were treated with or without dasatinib and grown in 3D culture (Materials and Methods). Then cells were stained with antibody to F-actin (green). Nucleus was counter stained with DAPI (blue). Scale bar = 10 µm