Table 2.
Epibiotic bacterial phylotypes recovered from diatom cells in culture and from natural waters and their closest cultivated relative identified by Blastn.
| Diatom host | na | Phylum/class | Order | Family | Species | 16S rRNA similarity (%) |
|---|---|---|---|---|---|---|
| Cultures | ||||||
| C. danicus RCC 2565 | 3 | Alphaproteobacteria | Rhodobacterales | Hyphomonadaceae | Algimonas sp. | 99.6 |
| 1 | Betaproteobacteria | Burkholderiales | Burkholderiaceae | Limnobacter thiooxidans | 100 | |
| 1 | Comamonadaceae | Pelomonas sp. | 99.9 | |||
| 1 | Gammaproteobacteria | Oceanospirillales | Halomonadaceae | Halomonas sp | 99.9 | |
| 1 | Flavobacteriia | Flavobacteriales | Cryomorphaceae | Brumimicrobium mesophilum | 93.5 | |
| 1 | Actinobacteria | Micrococcales | Micrococcaceae | Nesterenkonia flava | 99.4 | |
| 1 | Bacilli | Bacillales | Bacillaceae | Aeribacillus pallidus | 99.9 | |
| T. delicatula RCC 2560 | 1 | Alphaproteobacteria | Sphingomonadales | Erythrobacteraceae | Erythrobacter litoralis | 98.8 |
| 1 | Rhodobacterales | Rhodobacteraceae | Maribius pelagius | 94.7 | ||
| 1 | Bacilli | Bacillales | Bacillaceae | Aeribacillus pallidus | 100 | |
| 16 | Flavobacteriia | Flavobacteriales | Flavobacteriaceae | Tenacibaculum skagerrakense | 97.8 | |
| Environmental cellsb | ||||||
| Chaetoceros debilis | 1 | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | Marivita cryptomonadis | 98.5 |
| 1 | Pseudoruegeria lutimaris | 97 | ||||
| Chaetoceros protuberans | 1 | Gammaproteobacteria | Alteromonadales | Alteromonadaceae | Saccharophagus degradans | 95.1 |
| Thalassiosira punctigera | 1 | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae | Roseovarius nubinhibens | 97.9 |
| 1 | Gammaproteobacteria | Alteromonadales | Colwelliaceae | Colwellia psychroerythraea | 99.3 | |
| 1 | Chromatiales | Ectothiorhodospiraceae | Ectothiorhodospira mobilis | 94.3 | ||
| 1 | Cellvibrionales | Halieaceae | Chromatocurvus halotolerans | 95.7 | ||
| 1 | Spongiibacteraceae | Dasania marina | 93 | |||
| 1 | Oceanicoccus sagamiensis | 96.8 | ||||
| 1 | Pseudomonadales | Pseudomonadaceae | Pseudomonas trivialis | 90.7 | ||
| 4 | Cytophagia | Cytophagales | Amoebophilaceae | Candidatus Amoebophilus asiaticus | 93 | |
| 2 | Flavobacteriia | Flavobacteriales | Cryomorphaceae | Salinirepens amamiensis | 94.4 | |
| 1 | Sphingobacteriia | Sphingobacteriales | Saprospiraceae | Lewinella cohaerens | 89.1 |
aNumber of individual diatom cell carrying the corresponding bacterial phylotype; bas identified by 18S rRNA sequencing.