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. 2018 Dec 4;9:2950. doi: 10.3389/fmicb.2018.02950

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Copyright © 2018 Chen, Zheng, Yuan, Duan, Rong, Liu, Feng, Wang, Wang, Feng, Zhou, Li, Deng, Li, Xia, Rao, Zhou, Fu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CH6a full-length clone assembled from the consensus-like PCR fragments was more efficient in virus production. Two amino acids, S2362G in NS5A and N2738D in NS5B of CH6acc genome were mutated to the consensus sequence, designated CH6acc/Cons. RNA transcripts were transfected into Huh7.5 cells, and CH6acc was included in parallel. CH6acc/Cons was delayed in virus spread and attenuated in infectivity titers. HCV-positive cells were visualized by secure anti-HCV Core immunostaining (left y-axis; lines), and HCV infectivity titers were done by FFU assay (mean of triplicate infections ± SEM, right y-axis; bars). In other transfections, CH6aFL/Cons recombinant was non-viable.