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. 2018 Dec 4;9:1423. doi: 10.3389/fphar.2018.01423

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Copyright © 2018 Huang, Lan, Lu, Zhang, Zhang, Lou, Xu, Ren, Zhao, Sun, Li and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DiDang Tang inhibits ER stress-induced mitochondrial apoptosis in NGF-induced PC12 cells subjected to TM incubation. (A) After pretreatment with DDT or 4-PBA, followed by TM incubation, PC12 cells were stained with Annexin V-FITC/PI. We then detected the percentage of apoptotic cells by FCM. (B) Bar graph represents the number of apoptotic cells from (A) after the pretreatment with DDT or 4-PBA, followed by TM incubation. (C) Protein levels of Bcl-2, Bax, Cyto C, p-Bad, Bad and PARP were determined by Western blot. After the quantification by Image J, the intensity of each protein was normalized to the intensity of β-Actin and is shown on the right. ^∗P < 0.05 and ^∗∗∗P < 0.001 vs. Ctrl group; ^#P < 0.05 and ^###P < 0.001 vs. TM group (n = 3).