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. 2018 Nov 21;103(6):995–1008. doi: 10.1016/j.ajhg.2018.10.020

Figure 7.

Figure 7

DNAH9 DHC Domain 1 Interacts with CCDC114

Co-immunoprecipitation (co-IP) confirmed the interaction between DNAH9 fragment 2 and CCDC114. HEK293T cells were transfected with DNAH9-fragment-2-3xHA and CCDC114-3xFLAG (A), DNAH9 fragment-2-3xHA, and DYNLT3-3xFLAG (B, negative control). NPHP4-3xHA and RPGRIP1L-C-term-3xFLAG were used as positive control (C). Co-IP was performed with the anti-HA antibody while anti-FLAG beads were used for enrichment. Western blot (WB) was performed using an antibody directed against the FLAG tag. DNAH9-fragment-2 was able to precipitate full-length CCDC114 (A) with protein amounts detected in the co-IP comparable to what we observe for the positive control (C) while no interaction between DNAH9-fragment-2 and DYNLT3 was observed (negative control) (B). Lysate shows protein content before pull down, enrichment shows pulled down prey protein.