Fig. 4.
Cell cycle kinetics in corneal and limbal epithelia of mice. (A) Methodological scheme illustrating the 1.5-h interval between IdU and EdU injections in vivo. IdU-labelled cells red (A1), EdU-labelled green (A2). The red-only cells in (A3) marked with brown dots represent the Lcells. The red and green double-labelled cells in (A3) represent the Scells. Scale bars = 10 μm. (B) Average S-phase (Ts) and cell cycle time (Tc) (mean ± SEM) in epithelia of central cornea, peripheral cornea and limbus of 8 week and 12 month-old C57BL/6 mice. P < 0.05 (t-test; n = 6) is shown by the asterisk (*). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)