Fig. 2.

Proliferation of mNSC over 7 days. Growth curves (using the MTT assay) of cells grown as (A) a monolayer and (B) neurospheres, when cells were grown in growth factor-free medium (control), or in the presence of free- or dextrin-conjugated EGF and bFGF. Data represent mean ± SEM, n = 18. Categorization of neurospheres based upon diameter: small (100 μm), medium (100–200 μm) and large (> 200 μm) on (C) day 5 and (D) day 7. Data represent mean ± SEM, n = 6. Where error bars are invisible they are within size of data points. *Indicates significance p < .0001 compared to EGF + bFGF. Values above bars correspond to mean total neurosphere number ± SEM, n = 6.